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KMID : 0385220040140010033
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Korean Journal of Gerontology
2004 Volume.14 No. 1 p.33 ~ p.37
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Evidence for Presence of Thioredoxin Reductase in Nucleus of HeLa Cell
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Lee Seung-Rock
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Abstract
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Thioredoxin is an important cellular antioxidant and redox-regulator protein that functions as a primary line of defense against redox-regulated aging. It is regenerated after its action by thioredoxin reductase that has a selenocysteine residue essential for catalytic activity. Three distinct forms of human thioredoxin reductases reside in cytoplasm, mitochondrion, and microsome, respectively. However, thioredoxin reductase in nucleus was not identified. Whether thioredoxin reductase is present in nuclei of HeLa cells was investigated. The enzyme activity was estimated to be 0.056 mmoles/min/mg by spectrophotometric assay. The formation of reduced thioredoxin was confirmed by redox western blot assay. Either of the known forms of thioredoxin reductases was not detected in the nuclear extract. These results suggest that the nuclear enzyme is a new type of thioredoxin reductase.
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KEYWORD
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Thioredoxin reductase, Thioredoxin, Nucleus
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